重要提示：常規(guī)siRNA設(shè)計(jì)定制，堿基數(shù)19個默認(rèn)加懸垂，大于19個堿基需添加備注（是否加懸垂（dTdT））；化學(xué)修飾siRNA需添加備注修飾位置（3端或5端，正義鏈或反義鏈）。
產(chǎn)品優(yōu)勢
1、采用siCatch siRNA design智能設(shè)計(jì)；
2、通過系統(tǒng)性優(yōu)化，保證靶點(diǎn)特異性；
3、均經(jīng)過嚴(yán)格的質(zhì)譜檢測，保證siRNA質(zhì)量和純度；
4、只需溶解，即可直接用于實(shí)驗(yàn)。
哪種siRNA適合您？
單條siRNA不能保證沉默效率，初次篩選建議您訂購單基因套裝或者選購3-5條進(jìn)行實(shí)驗(yàn)，提升實(shí)驗(yàn)效率，加快實(shí)驗(yàn)進(jìn)度。
代表性客戶文章
- Shen L, Hu P, Zhang Y, et al. Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation[J]. Cell Metabolism, 2021.
- Sun B, Yang X, Hou F, et al. Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency[J]. Nature Microbiology, 2021: 1-15.
- Li S Y, Zhu Y, Li R N, et al. LncRNA Lnc‐APUE is Repressed by HNF4α and Promotes G1/S Phase Transition and Tumor Growth by Regulating MiR‐20b/E2F1 Axis[J]. Advanced Science, 2021: 2003094.
- Wang S, Zheng Y, Li J, et al. Single-cell transcriptomic atlas of primate ovarian aging[J]. Cell, 2020, 180(3): 585-600. e19.
- Lv H, Lv G, Chen C, et al. NAD+ metabolism maintains inducible PD-L1 expression to drive tumor immune evasion[J]. Cell Metabolism, 2021, 33(1): 110-127. e5.
- Tang C, Xie Y, Yu T, et al. m 6 A-dependent biogenesis of circular RNAs in male germ cells[J]. Cell research, 2020, 30(3): 211-228.
- Li Y, Xia L, Tan K, et al. N 6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2[J]. Nature Genetics, 2020, 52(9): 870-877.
- Shen Q, Chen Z, Zhao F, et al. Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1[J]. Nature Neuroscience, 2020, 23(7): 832-841.
- Dai J, Huang Y J, He X, et al. Acetylation blocks cGAS activity and inhibits self-DNA-induced autoimmunity[J]. Cell, 2019, 176(6): 1447-1460. e14.
- Dai P, Wang X, Gou L T, et al. A translation-activating function of MIWI/piRNA during mouse spermiogenesis[J]. Cell, 2019, 179(7): 1566-1581. e16.
- Zhang W, Wang G, Xu Z G, et al. Lactate is a natural suppressor of RLR signaling by targeting MAVS[J]. Cell, 2019, 178(1): 176-189. e15.
- Xu M, Xu H H, Lin Y, et al. LECT2, a ligand for Tie1, plays a crucial role in liver fibrogenesis[J]. Cell, 2019, 178(6): 1478-1492. e20.
技術(shù)支持
常規(guī)化學(xué)合成siRNA多為19nt RNA + dTdT (3’懸垂)的RNA雙鏈分子，未經(jīng)任何化學(xué)修飾。
Sense：正義鏈，RNA，序列與靶序列相同；
Antisense：反義鏈，RNA，序列與靶序列互補(bǔ)；
dTdT：兩端懸垂，DNA，均在3’端。
還支持各種特殊修飾或突變設(shè)計(jì)
圖1? 常規(guī)siRNA結(jié)構(gòu)示意圖
圖2 化學(xué)合成siRNA單鏈LC-MS質(zhì)控結(jié)果
訂購產(chǎn)品
| 產(chǎn)品編號 | 產(chǎn)品名稱 | 物種 | 目錄價(jià) | 數(shù)量 | 操作 |
|---|---|---|---|---|---|
| siB0837165937-1-5 | si-h-STAT3_002, Standard, 5nmol | human | ¥589.00 | ||
| siB160406112518-1-5 | si-m-Cd274_004, Standard, 5nmol | mouse | ¥589.00 | ||
| siB09917111357-1-5 | si-h-TP53_004, Standard, 5nmol | human | ¥589.00 | ||
| siB12127101403-1-5 | si-h-ALKBH5_001, Standard, 5nmol | human | ¥589.00 | ||
| siB12127101538-1-5 | si-h-ALKBH5_003, Standard, 5nmol | human | ¥589.00 | ||
| siB11331105721-1-5 | si-h-MYC_001, Standard, 5nmol | human | ¥589.00 | ||
| siB11331105730-1-5 | si-h-MYC_002, Standard, 5nmol | human | ¥589.00 | ||
| siG1909091202510020-1-5 | si-h-MNX1-AS1_002, Standard, 5nmol | human | ¥589.00 | ||
| siB09731120743-1-5 | si-h-TP53_003, Standard, 5nmol | human | ¥589.00 | ||
| siG2308310301584520-1-5 | si-m-Meak7_001, Standard, 5nmol | mouse | ¥589.00 |
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