外泌體提取試劑
外泌體(Exosome)功能研究的關(guān)鍵前提是提取出高質(zhì)量的外泌體,傳統(tǒng)提取方法利用超速離心或密度梯度離心對(duì)外泌體進(jìn)行富集,這些方法耗時(shí)耗力,提取效果或產(chǎn)量卻并不理想。
銳博生物擁有外泌體提取的自主專利技術(shù),提供新型Exosomes提取試劑盒Ribo??Exosome Isolation Reagent專門用于提取細(xì)胞上清、血清血漿或其他體液中的外泌體,僅需通過簡(jiǎn)單混勻和常規(guī)離心,即可從樣本中獲取大量結(jié)構(gòu)完整的Exosome,提供更加便捷、高效、高純度的提取方法,同時(shí)確保外泌體的結(jié)構(gòu)和功能完整性,提取產(chǎn)物可用于NGS、細(xì)胞共培養(yǎng)以及體內(nèi)注射等后續(xù)實(shí)驗(yàn)。
【一種分離外泌體的方法及其試劑盒? ?專利申請(qǐng)?zhí)枺篊A201510469340.3】
產(chǎn)品優(yōu)勢(shì)
1、操作簡(jiǎn)單,1小時(shí)快速抽提
2、廣泛兼容多種樣品:培養(yǎng)上清、血清/血漿、尿液、腦脊液、唾液、乳汁、肺泡灌洗液等
3、樣本少(血清2~4ml,培養(yǎng)上清20ml),提取量高
4、富集效果精準(zhǔn)可靠
技術(shù)支持

HeLa 細(xì)胞上清經(jīng)Ribo?Exosome Isolation Reagent 抽提,用攜帶熒光基團(tuán)的CD63 和CD81 抗體分別與抽提前樣本及抽提產(chǎn)物進(jìn)行免疫反應(yīng),使用流式細(xì)胞儀檢測(cè)熒光強(qiáng)度。結(jié)果表明,抽提前兩個(gè)標(biāo)志物抗體熒光強(qiáng)度均處于背景閾值范圍內(nèi),抽提后熒光強(qiáng)度明顯提升,呈現(xiàn)陽(yáng)性結(jié)果,表明exosomes 被富集出來。
代表性客戶文章
- Chen Q, Zhang F, Dong L, et al. SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs[J]. Cell Research, 2021, 31(3): 247-258.(細(xì)胞培養(yǎng)基)
- Sun L, Zhu W, Zhao P, et al. Long noncoding RNA UCA1 from hypoxia-conditioned hMSC-derived exosomes: a novel molecular target for cardioprotection through miR-873-5p/XIAP axis[J]. Cell death & disease, 2020, 11(8): 1-16.(細(xì)胞培養(yǎng)基、血清/血漿)
- Zhang K, Dong C, Chen M, et al. Extracellular vesicle-mediated delivery of miR-101 inhibits lung metastasis in osteosarcoma[J]. Theranostics, 2020, 10(1): 411.(血清/血漿)
- Liu X, Li X, Zhu W, et al. Exosomes from mesenchymal stem cells overexpressing MIF enhance myocardial repair[J]. Journal of Cellular Physiology, 2020.(細(xì)胞培養(yǎng)基)
- Bai M, Li J, Yang H, et al. MiR-135b delivered by gastric tumor exosomes inhibits FOXO1 expression in endothelial cells and promotes angiogenesis[J]. Molecular Therapy, 2019.(血清/血漿)
- ? Xu W, Wu Y, Hu Z, et al. Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity[J]. Molecular Therapy-Nucleic Acids, 2019, 16: 778-790.(細(xì)胞培養(yǎng)基)
訂購(gòu)產(chǎn)品
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 目錄價(jià) | 數(shù)量 | 操作 |
|---|---|---|---|---|
| C10130-1 | Exosome Isolation Reagent (for cell culture media), 15mL | ¥1,800.00 | ||
| C10120-1 | Exosome Isolation Reagent (for other body fluids) , 15mL | ¥1,800.00 | ||
| C10130-2 | Exosome Isolation Reagent (for cell culture media), 60mL | ¥5,900.00 | ||
| C10120-2 | Exosome Isolation Reagent (for other body fluids), 60mL | ¥5,900.00 | ||
| C10110-2 | Exosome Isolation Reagent (for plasma or serum), 10mL | ¥2,990.00 |
產(chǎn)品說明書下載
說明書為PDF文件。右鍵點(diǎn)擊,選擇保存鏈接即可下載。




